EAF2 Antikörper (AA 1-170)

Produktnummer ABIN6139905

Produktdetails anti-EAF2 Antikörper

Target: EAF2 (ELL Associated Factor 2 (EAF2))

Bindungsspezifität: AA 1-170

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EAF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Sequenz: MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY LEVGEGEQVT ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL EKLSSNITVK KTRVEGSSKI QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Polyclonal Antibodies

Aufreinigung: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human EAF2 (NP_060926.2).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB,1:500 - 1:2000,IF,1:50 - 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu EAF2

Target: EAF2 (ELL Associated Factor 2 (EAF2))

Andere Bezeichnung: EAF2 (EAF2 Produkte)

Synonyme: eaf2 antikoerper, MGC69052 antikoerper, MGC68453 antikoerper, zgc:86600 antikoerper, wu:fb76c01 antikoerper, wu:fc43g12 antikoerper, BM040 antikoerper, TRAITS antikoerper, U19 antikoerper, AW048865 antikoerper, FESTA-L antikoerper, FESTA-S antikoerper, Festa antikoerper, Traits antikoerper, ELL associated factor 2 S homeolog antikoerper, ELL associated factor 2 L homeolog antikoerper, ELL associated factor 2 antikoerper, eaf2.S antikoerper, eaf2.L antikoerper, eaf2 antikoerper, EAF2 antikoerper, Eaf2 antikoerper

Hintergrund: Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,EAF2,BM040,TRAITS,U19,Epigenetics & Nuclear Signaling,Cancer,Tumor suppressors,Cell Biology & Developmental Biology,Apoptosis,EAF2

Molekulargewicht: 14 kDa/28 kDa

Gen-ID: 55840

UniProt: Q96CJ1