This antibody reacts with C-terminal part of beta-Catenin, an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.
Kreuzreaktivität (Details)
Species reactivity (expected):Mouse, Hamster. Species reactivity (tested):Human.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH ~7.4, 15 mM Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.Synonyms: Beta-catenin, CTNNB, CTNNB1