S100A8/A9 Complex (Calprotectin) Antikörper (CF®594)
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- Target Alle S100A8/A9 Complex (Calprotectin) (S100A8/A9) Antikörper anzeigen
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
- Reaktivität
- Human, Rind (Kuh), Schwein, Cat, Hund, Meerschweinchen, Pferd, Affe, Kaninchen, Ratte, Pavian, Ziege, Maus
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser S100A8/A9 Complex (Calprotectin) Antikörper ist konjugiert mit CF®594
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Applikation
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-Calgranulin-B / MRP-8/-14 / MAC-387 / S100A8/A9 / Macrophage Ag (MAC387), CF594 Conjugate
- Produktmerkmale
- This antibody recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Affinity purified monocyte membrane preparation
- Klon
- MAC387
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product S100A8/A9 Primärantikörper
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- Applikationshinweise
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Tonsil, lymph node, or spleen
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (S100 Calcium Binding Protein A8/A9 (S100A8/A9))
- Andere Bezeichnung
- Calgranulin-B / MRP-8/-14 / MAC-387 / S100A8/A9 / Macrophage Ag (S100A8/A9 Produkte)
- Synonyme
- 60B8AG antikoerper, CAGB antikoerper, CFAG antikoerper, CGLB antikoerper, L1AG antikoerper, LIAG antikoerper, MAC387 antikoerper, MIF antikoerper, MRP14 antikoerper, NIF antikoerper, P14 antikoerper, S100 calcium binding protein A9 antikoerper, S100A9 antikoerper
- Molekulargewicht
- 12-14 kDa (doublet)
- Gen-ID
- 6279, 6280
- Pathways
- S100 Proteine
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