HLA-DP beta Antikörper (CF®488A)
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- Target Alle HLA-DP beta Produkte
- HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
- Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser HLA-DP beta Antikörper ist konjugiert mit CF®488A
- Applikation
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-MHC-II (HLA-DP beta chain) (BRA-FB6), CF488A Conjugate
- Produktmerkmale
- This antibody recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked i± (33-35 kDa) and i² (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
- Klon
- BRA-FB6
- Isotyp
- IgG2b kappa
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- Applikationshinweise
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Raji cells. Tonsil or lymph node
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- HLA-DP beta (MHC Class II HLA-DP beta (HLA-DP beta))
- Andere Bezeichnung
- MHC-II (HLA-DP) (beta chain) (HLA-DP beta Produkte)
- Molekulargewicht
- 33-35 kDa
- Gen-ID
- 3115, 347270
- UniProt
- P04440
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