TOP1MT Antikörper (CF®647)
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- Target Alle TOP1MT Antikörper anzeigen
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser TOP1MT Antikörper ist konjugiert mit CF®647
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Applikation
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-Topoisomerase-I, MT (TOP1MT/488), CF647 Conjugate
- Produktmerkmale
- DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant full-length human TOP1MT protein
- Klon
- TOP1MT-488
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product TOP1MT Primärantikörper
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- Applikationshinweise
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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A431 cells. Heart, Skeletal muscle, brain or fetal liver.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
- Andere Bezeichnung
- Topoisomerase-I, MT (TOP1MT Produkte)
- Synonyme
- 2900052H09Rik antikoerper, TOP1 antikoerper, topoisomerase (DNA) I, mitochondrial antikoerper, DNA topoisomerase 1, mitochondrial antikoerper, DNA topoisomerase I mitochondrial antikoerper, TOP1MT antikoerper, Top1mt antikoerper
- Hintergrund
- DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase
- Molekulargewicht
- 70 kDa
- Gen-ID
- 116447, 528574
- UniProt
- Q969P6
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