CD99 Antikörper (CF®647)
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- Target Alle CD99 Antikörper anzeigen
- CD99
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD99 Antikörper ist konjugiert mit CF®647
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Applikation
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-CD99 (MIC2/877), CF647 Conjugate
- Produktmerkmale
- This antibody recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant full-length human MIC2 protein
- Klon
- MIC2-877
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CD99 Primärantikörper
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- Applikationshinweise
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Immunohistology formalin-fixed 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Does not react with rat, others not tested
- Optimal dilution for a specific application should be determined by user
- Kommentare
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MOLT-4 cells. Pancreas or Ewing s sarcoma
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- CD99
- Andere Bezeichnung
- CD99 (CD99 Produkte)
- Synonyme
- 1110061M03Rik antikoerper, 2410026K10Rik antikoerper, D4 antikoerper, Pilr-l antikoerper, pilr-1 antikoerper, HBA71 antikoerper, MIC2 antikoerper, MIC2X antikoerper, MIC2Y antikoerper, MSK5X antikoerper, CD99 antigen antikoerper, CD99 molecule (Xg blood group) antikoerper, CD99 molecule antikoerper, Cd99 antikoerper, CD99 antikoerper
- Substanzklasse
- Viral Protein
- Hintergrund
- 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2
- Molekulargewicht
- 27-32 kDa
- Gen-ID
- 4267, 653349
- UniProt
- P14209
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