Keratin 10/13 Antikörper (Biotin)

Produktnummer ABIN6161006

Produktdetails

Target: Keratin 10/13

Reaktivität: Human, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Verwendungszweck: Mouse Monoclonal anti-Cytokeratin 10/13 (DE-K13), Biotin Conjugate

Produktmerkmale: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this MAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium

Klon: DE-K13

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.25-0.5 μg/mL

Kommentare:

A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Antigendetails

Target: Keratin 10/13

Andere Bezeichnung: Cytokeratin 10/13

Hintergrund: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10, CK13, K13, keratin type I cytoskeletal 13, Keratin-13, Krt1-13, KRT1

Molekulargewicht: 56.5 kDa (CK10) & 53 kDa (CK13)

Gen-ID: 3858, 99936

UniProt: P13645