KRT1 Antikörper (CF®647)
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- Target Alle KRT1 Antikörper anzeigen
- KRT1 (Cytokeratin HMW (KRT1))
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Reaktivität
- Human, Ratte, Maus, Kaninchen, Huhn, Rind (Kuh), Affe, Hund
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser KRT1 Antikörper ist konjugiert mit CF®647
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-Cytokeratin, HMW (AE-3), CF647 Conjugate
- Produktmerkmale
- This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Human epidermal keratin
- Klon
- AE-3
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product KRT1 Primärantikörper
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- Applikationshinweise
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Epithelial cells, Skin or Adenocarcinomas
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- KRT1 (Cytokeratin HMW (KRT1))
- Andere Bezeichnung
- Cytokeratin, HMW (KRT1 Produkte)
- Synonyme
- CK1 antikoerper, EHK antikoerper, EHK1 antikoerper, EPPK antikoerper, K1 antikoerper, KRT1A antikoerper, NEPPK antikoerper, keratin 1 antikoerper, KRT1 antikoerper
- Hintergrund
- KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
- Molekulargewicht
- 52-67 kDa
- Gen-ID
- 51350, 654392
- UniProt
- Q01546
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