TNF alpha Antikörper
-
- Target Alle TNF alpha Antikörper anzeigen
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
-
Reaktivität
- Human, Ratte
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser TNF alpha Antikörper ist unkonjugiert
-
Applikation
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Verwendungszweck
- Mouse Monoclonal anti-TNF-alpha (TNFA/1172), Purified, BSA-free
- Produktmerkmale
- This MAb recognizes human 17-26 kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Aufreinigung
- Purified
- Immunogen
- Recombinant full-length human TNF-alpha protein
- Klon
- TNFA-1172
- Isotyp
- IgM kappa
- Top Product
- Discover our top product TNF alpha Primärantikörper
-
-
- Applikationshinweise
-
Immunohistology formalin-paraffin 2-4 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
-
HeLa, HL-60, or A431 cells. Pancreas or Histiocytoma
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Konservierungsmittel
- Azide free
-
- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Andere Bezeichnung
- TNF-alpha (TNF alpha Produkte)
- Synonyme
- DIF antikoerper, TNF-alpha antikoerper, TNFA antikoerper, TNFSF2 antikoerper, RATTNF antikoerper, Tnfa antikoerper, tnf antikoerper, TNF-a antikoerper, TNFalpha antikoerper, Tnfsf1a antikoerper, TNFa antikoerper, cTNF antikoerper, Tnf-alpha antikoerper, tnfa-like antikoerper, TNF-ALPHA antikoerper, dif antikoerper, tnfa antikoerper, xtnf antikoerper, tnfsf2 antikoerper, tnf-alpha antikoerper, Cachectin antikoerper, tumor necrosis factor antikoerper, tumor necrosis factor b (TNF superfamily, member 2) antikoerper, tumor necrosis factor alpha antikoerper, tumor necrosis factor a (TNF superfamily, member 2) antikoerper, TNF antikoerper, Tnf antikoerper, tnf antikoerper, tnfb antikoerper, tnf-alpha antikoerper, LOC103694380 antikoerper, tnfa antikoerper
- Molekulargewicht
- 17 kDa
- Gen-ID
- 7124, 241570
- UniProt
- P01375
- Pathways
- NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
-