MLANA Antikörper (CF®568)
-
- Target Alle MLANA Antikörper anzeigen
- MLANA (Melan A (MLANA))
-
Reaktivität
- Human, Pferd
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser MLANA Antikörper ist konjugiert mit CF®568
-
Applikation
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-MART-1 / Melan-A (M2-7C10), CF568 Conjugate
- Produktmerkmale
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant hMART-1 protein
- Klon
- M2-7C10
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product MLANA Primärantikörper
-
-
- Applikationshinweise
-
Does not react with mouse or rat, others not tested
- Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
-
SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
-
- Target
- MLANA (Melan A (MLANA))
- Andere Bezeichnung
- MART-1 / Melan-A (MLANA Produkte)
- Synonyme
- A930034P04Rik antikoerper, Mart1 antikoerper, MART-1 antikoerper, MART1 antikoerper, melan-A antikoerper, Mlana antikoerper, MLANA antikoerper
- Hintergrund
- Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
- Molekulargewicht
- 20-22 kDa (doublet)
- Gen-ID
- 2315, 154069
- UniProt
- Q16655
-