Keratin 5/8 Antikörper (CF®568)
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- Target Alle Keratin 5/8 (KRT5/8) Produkte
- Keratin 5/8 (KRT5/8)
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Reaktivität
- Human, Maus, Schwein, Ratte, Hund, Hamster, Rind (Kuh), Schaf, Frettchen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Keratin 5/8 Antikörper ist konjugiert mit CF®568
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-Cytokeratin 5/8 (C-50), CF568 Conjugate
- Produktmerkmale
- This antibody recognizes keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Cytoskeletal preparation from HeLa cells
- Klon
- C-50
- Isotyp
- IgG1 kappa
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- Applikationshinweise
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Immunohistology formalin-fixed 0.5-1.0 μg/mL
- For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Western blotting 0.5-1.0 μg/mL
- Does not react with chicken , or Xenopus
- Optimal dilution for a specific application should be determined by user
- Kommentare
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HepG2 or A431 cells. Skin or Squamous cell carcinoma
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- Keratin 5/8 (KRT5/8)
- Andere Bezeichnung
- Cytokeratin 5/8 (KRT5/8 Produkte)
- Molekulargewicht
- 58 kDa (CK5), 52.5 kDa (CK8)
- Gen-ID
- 3852, 3856
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