Melanoma gp100 Antikörper (CF®405S)
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- Target Alle Melanoma gp100 (PMEL) Antikörper anzeigen
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Melanoma gp100 Antikörper ist konjugiert mit CF®405S
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Applikation
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- Mouse Monoclonal anti-Pmel17 / gp100 / SILV (HMB45), CF405S Conjugate
- Produktmerkmale
- By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Extract of pigmented melanoma metastases from lymph nodes
- Klon
- HMB45
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product PMEL Primärantikörper
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- Applikationshinweise
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Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Does not react with dog or rat, others not tested
- Optimal dilution for a specific application should be determined by user
- Kommentare
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SK-MEL-28 cells or Melanoma
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Andere Bezeichnung
- Pmel17 / gp100 / SILV (PMEL Produkte)
- Synonyme
- D12S53E antikoerper, ME20 antikoerper, ME20-M antikoerper, ME20M antikoerper, P1 antikoerper, P100 antikoerper, PMEL17 antikoerper, SI antikoerper, SIL antikoerper, SILV antikoerper, gp100 antikoerper, D10H12S53E antikoerper, D12S53Eh antikoerper, Pmel17 antikoerper, Si antikoerper, Silv antikoerper, gp87 antikoerper, RPE1 antikoerper, MMP115 antikoerper, silverb antikoerper, silver antikoerper, cb397 antikoerper, fdv antikoerper, pmel17 antikoerper, sb:cb397 antikoerper, silva antikoerper, wu:fc11g11 antikoerper, wu:fj24g11 antikoerper, zgc:136622 antikoerper, premelanosome protein antikoerper, premelanosome protein b antikoerper, premelanosome protein a antikoerper, PMEL antikoerper, Pmel antikoerper, pmelb antikoerper, pmela antikoerper
- Molekulargewicht
- 90-100 kDa
- Gen-ID
- 6490, 95972
- UniProt
- P40967
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