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CD73 Antikörper (APC)

Name: CD73 Antikörper (APC)
Brand: antibodies-online
SKU: ABIN6240819
Price: 439.92 EUR
Availability: InStock

NT5E Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal AD2 APC

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD73 (AD2) APC antibody (10 μL reagent / 100 μL of peripheral whole blood). (CD73 Antikörper (APC))

FACS

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD73 (AD2) APC antibody (10 μL reagent / 100 μL of peripheral whole blood) and anti-human CD19 (LT19) PE antibody(20 μL reagent / 100 μL of peripheral whole blood). (CD73 Antikörper (APC))

FACS

Separation of human CD73 positive CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD73 (AD2) APC antibody (10 μL reagent / 100 μL of peripheral whole blood). (CD73 Antikörper (APC))

FACS

3 Abbildungen

Produktnummer ABIN6240819

Datenblatt (PDF)

Target Alle CD73 (NT5E) Antikörper anzeigen

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
Reaktivität
118

69

31

18

18

16

3

2

1

1

1

1

1
Human, Non-Human Primate
Wirt
89

40

18

2
Maus
Passende Sekundärantikörper
Klonalität
79

69

1
Monoklonal
Konjugat
62

14

12

8

8

4

4

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD73 Antikörper ist konjugiert mit APC
Applikation
83

66

48

39

16

15

14

14

12

8

7

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1
Flow Cytometry (FACS)

Spezifität

The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.

Kreuzreaktivität (Details)

Human

Aufreinigung

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen

pre-B leukemia cells

Klon

AD2

Isotyp

IgG1 kappa

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anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)
NT5E Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal AD2 PE

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibody
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anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibody
NT5E Reaktivität: Human WB, IHC, IP, ICC Wirt: Maus Monoclonal D1 unconjugated

Applikationshinweise

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Kommentare

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Target

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Andere Bezeichnung

CD73 (NT5E Produkte)

Hintergrund

5'-nucleotidase ecto,CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.,ecto-5´nucleotidase, NT5E, E5NT, CALJA, NTE

Gen-ID

4907

UniProt

P21589

Pathways

Synaptic Membrane, Ribonucleoside Biosynthetic Process