RPA1 Antikörper

Produktnummer ABIN6241400

Dieses Maus Monoklonal-Antikörper erkennt spezifisch RPA1 in WB, IP und ICC. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für RPA1 Antikörper (ABIN6241400)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RPA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-RPA1 Antikörper

Immunogen: Recombinant Protein

Anwendungsinformationen

Applikationshinweise: IP: 1:500. WB: 1:1000. ICC: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C

Details zu RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Andere Bezeichnung: RPA70

Hintergrund: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:< a href="http://www.uniprot.org/citations/9430682" target="_blank">9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:< a href="http://www.uniprot.org/citations/24332808" target="_blank">24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:< a href="http://www.uniprot.org/citations/17765923" target="_blank">17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:< a href="http://www.uniprot.org/citations/7697716" target="_blank">7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:< a href="http://www.uniprot.org/citations/9765279" target="_blank">9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:< a href="http://www.uniprot.org/citations/17959650" target="_blank">17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2- containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:< a href="http://www.uniprot.org/citations/19996105" target="_blank">19996105).

UniProt: P27694

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA