Insulin Receptor Antikörper (AA 1325-1358)

Produktnummer ABIN6242226

Produktdetails anti-Insulin Receptor Antikörper

Target: Insulin Receptor (INSR)

Bindungsspezifität: AA 1325-1358

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Insulin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Mouse Insr antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1325-1358 amino acids from Mouse Insr.

Klon: RB50340

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000. WB: 1:2000. WB: 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Details zu Insulin Receptor

Target: Insulin Receptor (INSR)

Andere Bezeichnung: Insr (INSR Produkte)

Synonyme: CD220 antikoerper, HHF5 antikoerper, 4932439J01Rik antikoerper, D630014A15Rik antikoerper, IR antikoerper, IR-A antikoerper, IR-B antikoerper, 18402 antikoerper, CG18402 antikoerper, DIHR antikoerper, DILR antikoerper, DIR antikoerper, DIRH antikoerper, DIRbeta antikoerper, DInR antikoerper, DInr antikoerper, Dir-a antikoerper, Dir-b antikoerper, Dmel\\CG18402 antikoerper, INR antikoerper, INS antikoerper, Inr antikoerper, Inr-alpha antikoerper, Inr-beta antikoerper, InsR antikoerper, dINR antikoerper, dIR antikoerper, dIRH antikoerper, dInR antikoerper, dInr antikoerper, dInsR antikoerper, dinr antikoerper, dir antikoerper, er10 antikoerper, inr antikoerper, insulin/insulin-like growth factor receptor antikoerper, l(3)05545 antikoerper, l(3)93Dj antikoerper, l(3)er10 antikoerper, lnR antikoerper, ir-A antikoerper, CTK-1 antikoerper, ir antikoerper, INSR antikoerper, NV14476 antikoerper, cd220 antikoerper, hhf5 antikoerper, insulin receptor antikoerper, Insulin-like receptor antikoerper, insulin receptor L homeolog antikoerper, INSR antikoerper, Insr antikoerper, InR antikoerper, LOC100122567 antikoerper, LOC100451802 antikoerper, insr.L antikoerper

Hintergrund: Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src- homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti- apoptotic effect of insulin by inducing phosphorylation of BAD, regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K- AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). When present in a hybrid receptor with IGF1R, binds IGF1 (By similarity).

Molekulargewicht: 155610

UniProt: P15208

Pathways: NF-kappaB Signalweg, RTK Signalweg, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity