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Melanoma Antikörper

Reaktivität: Human, Pferd IF, IHC, FACS Wirt: Maus Monoclonal NKI-beteb unconjugated
Produktnummer ABIN6252230
  • Target Alle Melanoma Produkte
    Melanoma
    Reaktivität
    • 4
    • 2
    • 2
    • 1
    Human, Pferd
    Wirt
    • 4
    Maus
    Klonalität
    • 4
    Monoklonal
    Konjugat
    • 4
    Dieser Melanoma Antikörper ist unkonjugiert
    Applikation
    • 4
    • 3
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Spezifität
    Does not react with rat
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
    Klon
    NKI-beteb
    Isotyp
    IgG2b kappa
  • Applikationshinweise
    Flow Cytometry: 0.5-1 μg/million cells

    IF: 1-2 μg/mL

    IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

    Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target
    Melanoma
    Abstract
    Melanoma Produkte
    Hintergrund
    By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
    Gen-ID
    6490
    UniProt
    P40967
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