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Melanoma Antikörper

Reaktivität: Human, Hund, Maus IHC (p), IF, WB Wirt: Maus Monoclonal PNL2 unconjugated
Produktnummer ABIN6252231
  • Target Alle Melanoma Produkte
    Melanoma
    Reaktivität
    • 4
    • 2
    • 1
    • 1
    Human, Hund, Maus
    Wirt
    • 4
    Maus
    Klonalität
    • 4
    Monoklonal
    Konjugat
    • 4
    Dieser Melanoma Antikörper ist unkonjugiert
    Applikation
    • 3
    • 3
    • 2
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Western Blotting (WB)
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Melanocyte antigen was used as the immunogen for the Melanoma antibody PNL2.
    Klon
    PNL2
    Isotyp
    IgG1
  • Applikationshinweise
    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    Optimal dilution of the Melanoma antibody PNL2 should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target
    Melanoma
    Abstract
    Melanoma Produkte
    Hintergrund
    Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.
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