Not reactive in: Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942
Homologie
dicots including Glycine max, Salvia miltiorrhiza, Solanum tuberosum, deciduous flowering plant Populus alba
Produktmerkmale
Expected / apparent Molecular Weight of the Antigene: 9.25 kDa
Aufreinigung
serum
Immunogen
KLH-conjugated synthetic peptide chosen from PsbE protein of Arabidopsis thaliana P56779, AtCg00580
Applikationshinweise
1 : 5000 with standard ECL (WB)
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 100 μL of sterile water.
Handhabung
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Ido, Nield, Fukao, Nishimura, Sato, Ifuku: "Cross-linking evidence for multiple interactions of the PsbP and PsbQ proteins in a higher plant photosystem II supercomplex." in: The Journal of biological chemistry, Vol. 289, Issue 29, pp. 20150-7, (2014) (PubMed).
Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).
Shang, Yan, Liu, Cao, Mei, Xin, Wu, Wang, Du, Jiang, Zhang, Zhao, Sun, Liu, Yu, Zhang: "The Mg-Chelatase H Subunit of Arabidopsis Antagonizes a Group of Transcription Repressors to Relieve ABA-Responsive Genes of Inhibition." in: The Plant cell, (2010) (PubMed).
Target
PsbE
Hintergrund
AGI Code: ATCG00580 Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbF and is comprised of two low molecular mass polypeptides, a and h subunits, with molecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closely associated with PSII in all oxygenic photosynthetic organisms. The a and h subunits of the Cyt b559 are components of the minimal PSII reaction center complex that is still capable of primary charge separation In summary, both PsbE and PsbF are essential components for PSII assembly, and they are probably involved in electron transport mechanisms that help to protect PSII from photodamage.Altrnative protein name: PSII reaction center subunit V