RUNX1 Antikörper (pSer276)

Produktnummer ABIN6255152

Produktdetails anti-RUNX1 Antikörper

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Bindungsspezifität: pSer276

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RUNX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Phospho-RUNX1 / AML1 (Ser276) Antibody detects endogenous levels of RUNX1 / AML1 only when phosphorylated at Serine 276.

Homologie: Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human RUNX1 / AML1 around the phosphorylation site of Ser276.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Andere Bezeichnung: RUNX1 (RUNX1 Produkte)

Synonyme: RUNX1 antikoerper, runx1 antikoerper, AI462102 antikoerper, AML1 antikoerper, Cbfa2 antikoerper, Pebp2a2 antikoerper, Pebpa2b antikoerper, AML1-EVI-1 antikoerper, AMLCR1 antikoerper, CBFA2 antikoerper, EVI-1 antikoerper, PEBP2aB antikoerper, runxa antikoerper, Runx-1 antikoerper, XAML antikoerper, Xaml1 antikoerper, aml antikoerper, aml-1 antikoerper, aml1 antikoerper, aml1-evi-1 antikoerper, amlcr1 antikoerper, cbfa2 antikoerper, evi-1 antikoerper, pebp2ab antikoerper, Aml1 antikoerper, uncharacterized LOC473981 antikoerper, runt-related transcription factor antikoerper, runt related transcription factor 1 antikoerper, runt-related transcription factor 1 antikoerper, runt related transcription factor 1 L homeolog antikoerper, LOC473981 antikoerper, runt antikoerper, Runx1 antikoerper, RUNX1 antikoerper, runx1 antikoerper, runx1.L antikoerper

Hintergrund:

Description: Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis (PubMed:17431401). Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218). Inhibits KAT6B-dependent transcriptional activation (By similarity). Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells (By similarity).

Gene: RUNX1

Molekulargewicht: 50kDa

Gen-ID: 861

UniProt: Q01196