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LIM Domain Kinase 1 Antikörper (pThr508)

LIMK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

Western blot analysis of extracts from Hela cells,Hela+H2o2 cells and p-peptide Rat brain tissue, using Phospho-LIMK1 (Thr508) Antibody(af3345) at 1/1000 dilution. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human gastric tissue sections by ICC/IF. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human heart tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat gastric tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat liver tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat tumor tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining mouse splenic tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining Mouse spleen tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human lymphoma tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat lung tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining mouse testicular tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human liver tissue cells by ICC/IF. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human renal clear cell carcinoma tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human colon cancer tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human seminoma tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat appendiceal tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining mouse lung tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining mouse pancreatic tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining Human lung cancer tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining Human lung cancer tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat kidney tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat uterine tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human brain tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining mouse gastric tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human lung cancer tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 staining NIH-3T3 by IF/ICC. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human gastric tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat thymus tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human vascular cancer tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat splenic tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human esophageal carcinoma tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat brain tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human liver cancer tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining human duodenum tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat testicular tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/200 staining Mouse spleen tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat heart tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat ovarian tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining rat intestinal tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining mouse kidney tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 at 1/100 staining mouse brain tissue sections by IHC-P. (LIM Domain Kinase 1 Antikörper (pThr508))

Western blot analysis of LIMK1 phosphorylation expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (LIM Domain Kinase 1 Antikörper (pThr508))

ABIN6267554 staining NIH-3T3 by IF/ICC. (LIM Domain Kinase 1 Antikörper (pThr508))

43 Abbildungen

Produktnummer ABIN6255701

Datenblatt (PDF)

Target Alle LIM Domain Kinase 1 (LIMK1) Antikörper anzeigen

LIM Domain Kinase 1 (LIMK1)
Bindungsspezifität
23

15

15

8

7

7

5

5

4

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr508
Reaktivität
107

78

73

2

2

1

1

1

1

1
Human, Maus, Ratte
Wirt
101

21

3
Kaninchen
Passende Sekundärantikörper
Klonalität
105

20
Polyklonal
Konjugat
59

9

8

5

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2
Dieser LIM Domain Kinase 1 Antikörper ist unkonjugiert
Applikation
90

48

26

26

24

19

16

8

7

6

6

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität

Phospho-LIMK1 (Thr508) Antibody detects endogenous levels of LIMK1 only when phosphorylated at Threonine 508.

Homologie

Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen

A synthesized peptide derived from human LIMK1 around the phosphorylation site of Thr508.

Isotyp

IgG
anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 Reaktivität: Human, Maus, Ratte, Affe WB, IHC, IF, IHC (p), FACS Wirt: Maus Monoclonal 3H6 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 Reaktivität: Human, Maus, Ratte, Hund, Affe WB, IHC, IF, IHC (p), FACS Wirt: Maus Monoclonal 6B4 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 451-550) antibody
LIMK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 Reaktivität: Human WB, ELISA, IHC (p) Wirt: Maus Monoclonal 1B2 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 1A8 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 Reaktivität: Human WB, ELISA, IHC (p) Wirt: Maus Monoclonal 2E9 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 Reaktivität: Human WB, ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 1-647) antibody
LIMK1 Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 1-30), (N-Term) antibody
LIMK1 Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal RB3078 unconjugated

Applikationshinweise

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500, ELISA(peptide) 1:20000-1:40000

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 mg/mL

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit

12 months
Target

LIM Domain Kinase 1 (LIMK1)

Andere Bezeichnung

LIMK1 (LIMK1 Produkte)

Synonyme

limk1 antikoerper, xlimk1 antikoerper, GB16654 antikoerper, LIMK1 antikoerper, LIMK antikoerper, LIMK-1 antikoerper, LIM domain kinase 1 antikoerper, LIM domain kinase 1a antikoerper, LIM domain kinase 1 L homeolog antikoerper, LIM-domain containing, protein kinase antikoerper, LIMK1 antikoerper, limk1a antikoerper, limk1 antikoerper, CpipJ_CPIJ000717 antikoerper, LOC413152 antikoerper, Limk1 antikoerper, limk1.L antikoerper

Hintergrund

Description: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
Gene: LIMK1

Molekulargewicht

70kDa

Gen-ID

3984

UniProt

P53667

Pathways

Caspase Kaskade in der Apoptose, Regulation of Cell Size, CXCR4-mediated Signaling Events

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