RPS6KB1 Antikörper (pSer424)

Produktnummer ABIN6256333

Produktdetails anti-RPS6KB1 Antikörper

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Bindungsspezifität: pSer424

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPS6KB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Phospho-p70 S6 Kinase (Ser424) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Ser447, which site historically referenced as Ser424.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Ser424.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Andere Bezeichnung: RPS6KB1 (RPS6KB1 Produkte)

Synonyme: PS6K antikoerper, S6K antikoerper, S6K-beta-1 antikoerper, S6K1 antikoerper, STK14A antikoerper, p70 S6KA antikoerper, p70(S6K)-alpha antikoerper, p70-S6K antikoerper, p70-alpha antikoerper, 10539 antikoerper, 7084 antikoerper, CG10539 antikoerper, DS6K antikoerper, Dmel\\CG10539 antikoerper, Dp70S6k antikoerper, Dp70[s6k] antikoerper, Dp70s6k antikoerper, S6k antikoerper, dS6K antikoerper, dS6k antikoerper, dp70[S6k] antikoerper, dp70s6k antikoerper, dps6k antikoerper, ds6k antikoerper, fs(3)07084 antikoerper, l(3)07084 antikoerper, p70 S6K antikoerper, p70/S6K antikoerper, p70S6K antikoerper, p70[S6 kinase] antikoerper, p70[S6K] antikoerper, p70[S6k] antikoerper, p70[S6kinase] antikoerper, p70s6K antikoerper, s6k antikoerper, s6k11 antikoerper, 2610318I15Rik antikoerper, 4732464A07Rik antikoerper, 70kDa antikoerper, AA959758 antikoerper, AI256796 antikoerper, AI314060 antikoerper, p70/85s6k antikoerper, p70s6k antikoerper, p70s6k-A antikoerper, p70-s6k antikoerper, ps6k antikoerper, rps6kb1 antikoerper, rps6kb1-A antikoerper, s6K1 antikoerper, stk14a antikoerper, fc51h01 antikoerper, wu:fc51h01 antikoerper, zgc:55713 antikoerper, ribosomal protein S6 kinase B1 antikoerper, Ribosomal protein S6 kinase antikoerper, ribosomal protein S6 kinase, polypeptide 1 antikoerper, ribosomal protein S6 kinase B1 L homeolog antikoerper, ribosomal protein S6 kinase B1 S homeolog antikoerper, ribosomal protein S6 kinase b, polypeptide 1b antikoerper, RPS6KB1 antikoerper, S6k antikoerper, Rps6kb1 antikoerper, rps6kb1.L antikoerper, rps6kb1.S antikoerper, rps6kb1b antikoerper

Hintergrund:

Description: Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR (PubMed:11500364, PubMed:12801526, PubMed:14673156, PubMed:15071500, PubMed:15341740, PubMed:16286006, PubMed:17052453, PubMed:17053147, PubMed:17936702, PubMed:18952604, PubMed:19085255, PubMed:19720745, PubMed:19935711, PubMed:19995915, PubMed:23429703). Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition (PubMed:28178239).

Gene: RPS6KB1

Molekulargewicht: 70kDa

Gen-ID: 6198

UniProt: P23443

Pathways: PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effekt