MED1 Antikörper (pThr1457)

Produktnummer ABIN6256479

Produktdetails anti-MED1 Antikörper

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Bindungsspezifität: pThr1457

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Phospho-TRAP220/MED1 (Thr1457) Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Threonine 1457.

Homologie: Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human TRAP220/MED1 around the phosphorylation site of Thr1457.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Andere Bezeichnung: MED1 (MED1 Produkte)

Synonyme: CRSP1 antikoerper, CRSP200 antikoerper, DRIP205 antikoerper, DRIP230 antikoerper, PBP antikoerper, PPARBP antikoerper, PPARGBP antikoerper, RB18A antikoerper, TRAP220 antikoerper, TRIP2 antikoerper, CRSP-1 antikoerper, MED1 antikoerper, pbp antikoerper, nbr1 antikoerper, crsp1 antikoerper, rb18a antikoerper, trip2 antikoerper, pparbp antikoerper, crsp200 antikoerper, drip205 antikoerper, drip230 antikoerper, ppargbp antikoerper, trap220 antikoerper, CG7162 antikoerper, Dmel\\CG7162 antikoerper, Med1 antikoerper, Med1/SOP3/TRAP220 antikoerper, Trap220 antikoerper, dTRAP220 antikoerper, AI480703 antikoerper, CRSP210 antikoerper, Pparbp antikoerper, l11Jus15 antikoerper, RGD1559552 antikoerper, mediator complex subunit 1 antikoerper, calcitonin-related polypeptide beta antikoerper, Mediator complex subunit 1 antikoerper, mediator of RNA polymerase II transcription subunit 1 antikoerper, MED1 antikoerper, CALCB antikoerper, med1 antikoerper, Med1 antikoerper

Hintergrund:

Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).

Gene: MED1

Molekulargewicht: 168kDa

Gen-ID: 5469

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha