IRAK4 Antikörper (pSer346, pThr345)

Produktnummer ABIN6256548

Produktdetails anti-IRAK4 Antikörper

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Bindungsspezifität: pSer346, pThr345

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRAK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Spezifität: Phospho-IRAK4 (Thr345/Ser346) Antibody detects endogenous levels of IRAK4 only when phosphorylated at Thr345/Ser346.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human IRAK4 around the phosphorylation site of Thr345/Ser346.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Andere Bezeichnung: IRAK4 (IRAK4 Produkte)

Synonyme: zgc:55553 antikoerper, IRAK4 antikoerper, ipd1 antikoerper, irak-4 antikoerper, ny-ran-64 antikoerper, ny-ren-64 antikoerper, ren64 antikoerper, irak4 antikoerper, IPD1 antikoerper, IRAK-4 antikoerper, NY-REN-64 antikoerper, REN64 antikoerper, 8430405M07Rik antikoerper, 9330209D03Rik antikoerper, interleukin 1 receptor associated kinase 4 antikoerper, interleukin-1 receptor-associated kinase 4 antikoerper, IRAK4 antikoerper, irak4 antikoerper, Irak4 antikoerper

Hintergrund:

Description: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Gene: IRAK4

Molekulargewicht: 60 kDa

Gen-ID: 51135

UniProt: Q9NWZ3

Pathways: NF-kappaB Signalweg, TLR Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades