E2F1 Antikörper (pThr433)

Produktnummer ABIN6256563

Produktdetails anti-E2F1 Antikörper

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Bindungsspezifität: pThr433

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Phospho-E2F1 (Thr433) Antibody detects endogenous levels of E2F1 only when phosphorylated at Threonine 433.

Homologie: Pig,Bovine,Horse,Rabbit,Chicken

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Andere Bezeichnung: E2F1 (E2F1 Produkte)

Synonyme: CG6376 antikoerper, DRTF1/E2F antikoerper, DmE2F-1 antikoerper, Dmel\\CG6376 antikoerper, Dp antikoerper, E(Sev-CycE)3A antikoerper, E(var)3-93E antikoerper, E(var)3-95E antikoerper, E(var)93E antikoerper, E2F antikoerper, E2F-1 antikoerper, E2F1 antikoerper, E2f1 antikoerper, Evar(3)164 antikoerper, dE2F antikoerper, dE2F1 antikoerper, dE2f antikoerper, dE2f1 antikoerper, de2f1 antikoerper, def21 antikoerper, drosE2F1 antikoerper, e2f antikoerper, e2f1 antikoerper, l(3)07172 antikoerper, l(3)j3B1 antikoerper, l(3)j3C2 antikoerper, l(3)rM729 antikoerper, RBAP1 antikoerper, RBBP3 antikoerper, RBP3 antikoerper, mKIAA4009 antikoerper, xE2F antikoerper, ATE2FB antikoerper, E2F TRANSCRIPTION FACTOR-1 E2F1 antikoerper, E2F transcription factor 1 antikoerper, E2FB antikoerper, E2FB TRANSCRIPTION FACTOR antikoerper, E2F transcription factor 1 antikoerper, E2F transcription factor 1 L homeolog antikoerper, E2f1 antikoerper, E2F1 antikoerper, e2f1.L antikoerper

Hintergrund:

Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). Positively regulates transcription of RRP1B (PubMed:20040599).

Gene: E2F1

Molekulargewicht: 47kDa

Gen-ID: 1869

UniProt: Q01094

Pathways: p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie