Topoisomerase I Antikörper (Internal Region)

Produktnummer ABIN6257133

Produktdetails anti-Topoisomerase I Antikörper

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: TOP1 Antibody detects endogenous levels of total TOP1.

Homologie: Pig,Zebrafish,Horse,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TOP1, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: TOP1 (TOP1 Produkte)

Synonyme: CG6146 antikoerper, Dmel\\CG6146 antikoerper, TOP1 antikoerper, TopI antikoerper, Topo I antikoerper, TopoI antikoerper, dTopoI antikoerper, l(1)G0134 antikoerper, l(1)G0201 antikoerper, l(1)G0229 antikoerper, l(1)G0278 antikoerper, l(1)top1 antikoerper, top1 antikoerper, topo I antikoerper, topoI antikoerper, DNA topoisomerase 1 beta antikoerper, DNA topoisomerase I alpha antikoerper, MGO1 antikoerper, MGOUN 1 antikoerper, MTE17.1 antikoerper, MTE17_1 antikoerper, TOP1BETA antikoerper, TOPOISOMERASE 1 antikoerper, TOPI antikoerper, AI467334 antikoerper, D130064I21Rik antikoerper, Top-1 antikoerper, Ab2-086 antikoerper, topi antikoerper, Topoisomerase 1 antikoerper, DNA topoisomerase I antikoerper, DNA topoisomerase I alpha antikoerper, DNA topoisomerase 1 antikoerper, topoisomerase (DNA) I antikoerper, DNA topoisomerase I, gene 1 L homeolog antikoerper, Top1 antikoerper, top1 antikoerper, topA antikoerper, ZMO1193 antikoerper, MRAD2831_RS63095 antikoerper, Lferr_0016 antikoerper, PC1_2015 antikoerper, Za10_0136 antikoerper, Gbro_0575 antikoerper, Dd586_2108 antikoerper, Kvar_3044 antikoerper, Alvin_2069 antikoerper, Dacet_0849 antikoerper, Mrub_1615 antikoerper, Arnit_2962 antikoerper, Ndas_4953 antikoerper, Trad_2377 antikoerper, Acear_1653 antikoerper, Mahau_0844 antikoerper, Mesop_4109 antikoerper, Thein_1586 antikoerper, Theth_0241 antikoerper, TOP1ALPHA antikoerper, top-1 antikoerper, TOP1 antikoerper, top1.1.L antikoerper

Substanzklasse: Viral Protein

Hintergrund:

Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Gene: TOP1

Molekulargewicht: 90 kDa

Gen-ID: 7150

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance