EIF3F Antikörper (Internal Region)

Produktnummer ABIN6257159

Produktdetails anti-EIF3F Antikörper

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF3F Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: EIF3F Antibody detects endogenous levels of total EIF3F.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EIF3F, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu EIF3F

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Andere Bezeichnung: EIF3F (EIF3F Produkte)

Synonyme: AO090005001590 antikoerper, Dmel\CG9769 antikoerper, eIF3-S5 antikoerper, eif3f antikoerper, LOC733116 antikoerper, eIF3f antikoerper, EIF3S5 antikoerper, eIF3-p47 antikoerper, 0610037M02Rik antikoerper, AA409853 antikoerper, Eif3s5 antikoerper, eukaryotic translation initiation factor 3 subunit F antikoerper, hypothetical protein antikoerper, CG9769 gene product from transcript CG9769-RA antikoerper, eukaryotic translation initiation factor 3 subunit 5 antikoerper, eukaryotic translation initiation factor 3, subunit F antikoerper, Eukaryotic translation initiation factor 3 subunit F antikoerper, eif3f antikoerper, ANI_1_120074 antikoerper, AOR_1_2780174 antikoerper, PTRG_03897 antikoerper, PAAG_00376 antikoerper, MCYG_08502 antikoerper, MGYG_08653 antikoerper, PGTG_04587 antikoerper, CG9769 antikoerper, LOC733116 antikoerper, EIF3F antikoerper, Eif3f antikoerper, eif-3.F antikoerper

Hintergrund:

Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Gene: EIF3F

Molekulargewicht: 38 kDa

Gen-ID: 8665

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization