NCBP2 Antikörper (N-Term)

Produktnummer ABIN6257882

Produktdetails anti-NCBP2 Antikörper

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCBP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: NCBP2 Antibody detects endogenous levels of total NCBP2.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NCBP2, corresponding to a region within N-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu NCBP2

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Andere Bezeichnung: NCBP2 (NCBP2 Produkte)

Synonyme: CBP20 antikoerper, CG12357 antikoerper, Dmel\\CG12357 antikoerper, 20kDa antikoerper, 5930413E18Rik antikoerper, AA536802 antikoerper, AI843301 antikoerper, C79367 antikoerper, CHUNP6917 antikoerper, zgc:92592 antikoerper, CBC2 antikoerper, NIP1 antikoerper, ATCBP20 antikoerper, CAP-binding protein 20 antikoerper, MLN1.12 antikoerper, MLN1_12 antikoerper, Ncbp2 antikoerper, cap binding protein 20 antikoerper, nuclear cap binding protein subunit 2 antikoerper, nuclear cap binding protein subunit 2, 20kDa antikoerper, nuclear cap binding protein subunit 2 L homeolog antikoerper, nuclear cap-binding protein subunit 2 antikoerper, CAP-binding protein 20 antikoerper, Nuclear cap-binding protein subunit 2 antikoerper, Cbp20 antikoerper, Ncbp2 antikoerper, ncbp2 antikoerper, NCBP2 antikoerper, ncbp2.L antikoerper, CBP20 antikoerper, ncbp-2 antikoerper

Hintergrund:

Description: Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (PubMed:26382858).

Gene: NCBP2

Molekulargewicht: 18 kDa

Gen-ID: 22916

UniProt: P52298

Pathways: Ribonucleoprotein Complex Subunit Organization, Methionine Biosynthetic Process