CELF1 Antikörper (N-Term)

Produktnummer ABIN6258447

Produktdetails anti-CELF1 Antikörper

Target: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CELF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: CELF-1 Antibody detects endogenous levels of total CELF-1.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CELF-1, corresponding to a region within N-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu CELF1

Target: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Andere Bezeichnung: CELF1 (CELF1 Produkte)

Synonyme: CUGBP1 antikoerper, BRUNOL2 antikoerper, CUG-BP antikoerper, CUGBP antikoerper, EDEN-BP antikoerper, NAB50 antikoerper, NAPOR antikoerper, hNab50 antikoerper, 1600010O03Rik antikoerper, AA407467 antikoerper, Brunol2 antikoerper, CUG-BP1 antikoerper, Cugbp1 antikoerper, D2Wsu101e antikoerper, HNAB50 antikoerper, Bruno-like antikoerper, brul antikoerper, cb920 antikoerper, cugbp1 antikoerper, brunol2 antikoerper, cug-bp antikoerper, cug-bp1 antikoerper, cugbp antikoerper, cugbp1-a antikoerper, eden-bp antikoerper, edenbp antikoerper, nab50 antikoerper, CUGBP Elav-like family member 1 antikoerper, CUGBP, Elav-like family member 1 antikoerper, cugbp, Elav-like family member 1 antikoerper, CUGBP Elav-like family member 1 L homeolog antikoerper, CELF1 antikoerper, Celf1 antikoerper, celf1 antikoerper, celf1.L antikoerper

Hintergrund:

Description: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF2, negatively regulates the processing to mature miRNA (PubMed:28431233).

Gene: CELF1

Molekulargewicht: 52 kDa

Gen-ID: 10658

UniProt: Q92879

Pathways: Ribonucleoprotein Complex Subunit Organization