OCT4 Antikörper (AA 114-163)

Produktnummer ABIN625854

Produktdetails anti-OCT4 Antikörper

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Bindungsspezifität: AA 114-163

Reaktivität: Human, Schwein, Kaninchen, Ziege

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human POU5F1 / OCT4

Homologie: Percent identity by BLAST analysis: Human, Goat (100%) Rabbit (92%) Dog (91%) Pig (84%).

Aufreinigung: Protein A/G purified

Immunogen: Synthetic peptide located between aa114-163 of human POU5F1 (Q01860, NP_002692). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Goat, Rabbit (100%), Marmoset (92%), Dog (91%), Bovine, Pig, Guinea pig (85%), Horse (84%), Cat (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1.25 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: PBS, 2 % sucrose.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: POU5F1 / OCT4 (POU5F1 Produkte)

Synonyme: OCT3 antikoerper, OCT4 antikoerper, OTF-3 antikoerper, OTF3 antikoerper, OTF4 antikoerper, Oct-3 antikoerper, Oct-4 antikoerper, POU5F1 antikoerper, gp9 antikoerper, gp-9 antikoerper, oct4 antikoerper, pou2 antikoerper, POU-2 antikoerper, cb197 antikoerper, spg/pou2 antikoerper, chunp6868 antikoerper, wu:fd18d06 antikoerper, NF-A3 antikoerper, Oct-3/4 antikoerper, Oct3 antikoerper, Oct3/4 antikoerper, Oct4 antikoerper, Otf-3 antikoerper, Otf-4 antikoerper, Otf3 antikoerper, Otf3-rs7 antikoerper, Otf3g antikoerper, Otf4 antikoerper, POU class 5 homeobox 1 antikoerper, POU domain, class 5, transcription factor 3 antikoerper, POU domain, class 5, transcription factor 1 antikoerper, POU5F1 antikoerper, pou5f3 antikoerper, Pou5f1 antikoerper

Hintergrund: Name/Gene ID: POU5F1

Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3

Gen-ID: 5460

NCBI Accession: NP_002692

UniProt: Q01860

Pathways: Stem Cell Maintenance