OCT4 Antikörper (AA 114-163)
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- Target Alle OCT4 (POU5F1) Antikörper anzeigen
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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Bindungsspezifität
- AA 114-163
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Reaktivität
- Human, Schwein, Kaninchen, Ziege
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser OCT4 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human POU5F1 / OCT4
- Homologie
- Percent identity by BLAST analysis: Human, Goat (100%) Rabbit (92%) Dog (91%) Pig (84%).
- Aufreinigung
- Protein A/G purified
- Immunogen
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Synthetic peptide located between aa114-163 of human POU5F1 (Q01860, NP_002692). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Goat, Rabbit (100%), Marmoset (92%), Dog (91%), Bovine, Pig, Guinea pig (85%), Horse (84%), Cat (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product POU5F1 Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (5 μg/mL), WB (1.25 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Rekonstitution
- Distilled water
- Konzentration
- Lot specific
- Buffer
- PBS, 2 % sucrose.
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Andere Bezeichnung
- POU5F1 / OCT4 (POU5F1 Produkte)
- Hintergrund
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Name/Gene ID: POU5F1
Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3 - Gen-ID
- 5460
- NCBI Accession
- NP_002692
- UniProt
- Q01860
- Pathways
- Stem Cell Maintenance
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