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MHC Class II RT-1B Antikörper

Reaktivität: Ratte, Maus FACS, IHC (fro), IHC (p), IHC, WB Wirt: Maus Monoclonal OX-6 unconjugated
Produktnummer ABIN625901
  • Target Alle MHC Class II RT-1B Produkte
    MHC Class II RT-1B
    Reaktivität
    Ratte, Maus
    Wirt
    • 8
    Maus
    Klonalität
    • 8
    Monoklonal
    Konjugat
    • 2
    • 2
    • 2
    • 2
    Dieser MHC Class II RT-1B Antikörper ist unkonjugiert
    Applikation
    • 8
    • 3
    • 2
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
    Marke
    IHC-plus™
    Spezifität
    Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.
    Aufreinigung
    Protein G purified
    Immunogen
    Rat thymocyte membrane glycoprotein.

    Type of Immunogen: Membrane extract
    Klon
    OX-6
    Isotyp
    IgG1
  • Applikationshinweise
    Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB

    Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer  pH 6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)
    Kommentare

    Target Species of Antibody: Rat

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS,  pH 7.2, 0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    MHC Class II RT-1B
    Abstract
    MHC Class II RT-1B Produkte
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