CHCHD4 Antikörper (N-Term)

Produktnummer ABIN6260791

Produktdetails anti-CHCHD4 Antikörper

Target: CHCHD4 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 4 (CHCHD4))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHCHD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: CHCHD4 Antibody detects endogenous levels of total CHCHD4.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CHCHD4, corresponding to a region within N-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu CHCHD4

Target: CHCHD4 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 4 (CHCHD4))

Andere Bezeichnung: CHCHD4 (CHCHD4 Produkte)

Synonyme: 2410012P20Rik antikoerper, 2810014D17Rik antikoerper, AI838740 antikoerper, MIA40 antikoerper, TIMM40 antikoerper, mia40 antikoerper, timm40 antikoerper, chchd4 antikoerper, chchd4a antikoerper, fc10g04 antikoerper, wu:fc10g04 antikoerper, zgc:100849 antikoerper, si:dkey-202e22.1 antikoerper, chchd4b antikoerper, CaO19.2977 antikoerper, coiled-coil-helix-coiled-coil-helix domain containing 4 antikoerper, coiled-coil-helix-coiled-coil-helix domain containing 4 L homeolog antikoerper, coiled-coil-helix-coiled-coil-helix domain containing 4a antikoerper, coiled-coil-helix-coiled-coil-helix domain containing 4 S homeolog antikoerper, Mia40p antikoerper, Chchd4 antikoerper, CHCHD4 antikoerper, chchd4 antikoerper, chchd4.L antikoerper, chchd4a antikoerper, chchd4.S antikoerper, LOC100361898 antikoerper, MIA40 antikoerper

Hintergrund:

Description: Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19 and MICU1 (PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364, PubMed:23676665). Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (PubMed:16185709, PubMed:19182799, PubMed:21059946, PubMed:23676665). Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:23186364). Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake (PubMed:26387864).

Gene: CHCHD4

Molekulargewicht: 22 kDa

Gen-ID: 131474

UniProt: Q8N4Q1