Cyclin D2 Antikörper (C-Term)

Produktnummer ABIN6261147

Der Kaninchen Polyklonal anti-Cyclin D2 Antikörper wird verwendet zum Nachweis von Cyclin D2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF und ICC.

Kurzübersicht für Cyclin D2 Antikörper (C-Term) (ABIN6261147)

Target: Cyclin D2 (CCND2)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin D2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-Cyclin D2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Cyclin D2 Antibody

Spezifität: Cyclin D2 Antibody detects endogenous levels of total Cyclin D2.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Cyclin D2, corresponding to a region within C-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu Cyclin D2

Target: Cyclin D2 (CCND2)

Andere Bezeichnung: Cyclin D2

Hintergrund:

Description: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Gene: CCND2

Molekulargewicht: 33 kDa

Gen-ID: 894

UniProt: P30279

Pathways: Zellzyklus, Mitotic G1-G1/S Phases