DCLRE1C Antikörper (C-Term)

Produktnummer ABIN6261238

Produktdetails anti-DCLRE1C Antikörper

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DCLRE1C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: DCLRE1C Antibody detects endogenous levels of total DCLRE1C.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DCLRE1C, corresponding to a region within C-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu DCLRE1C

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Andere Bezeichnung: DCLRE1C (DCLRE1C Produkte)

Synonyme: artemis antikoerper, A-SCID antikoerper, DCLREC1C antikoerper, RS-SCID antikoerper, SCIDA antikoerper, SNM1C antikoerper, hSNM1C antikoerper, Snm1l antikoerper, nuclease antikoerper, 9930121L06Rik antikoerper, AI661365 antikoerper, Art antikoerper, DNA cross-link repair 1C antikoerper, DCLRE1C antikoerper, Dclre1c antikoerper

Hintergrund:

Description: Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.

Gene: DCLRE1C

Molekulargewicht: 78kDa

Gen-ID: 64421

UniProt: Q96SD1

Pathways: DNA Reparatur