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DGCR8 Antikörper (C-Term)

DGCR8 Reaktivität: Human, Maus, Ratte WB, ELISA, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6261282
  • Target Alle DGCR8 Antikörper anzeigen
    DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
    Bindungsspezifität
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 44
    • 15
    • 13
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 38
    • 6
    Kaninchen
    Klonalität
    • 28
    • 16
    Polyklonal
    Konjugat
    • 32
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser DGCR8 Antikörper ist unkonjugiert
    Applikation
    • 34
    • 17
    • 10
    • 9
    • 8
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Spezifität
    DGCR8 Antibody detects endogenous levels of total DGCR8.
    Homologie
    Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus
    Aufreinigung
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human DGCR8, corresponding to a region within C-terminal amino acids.
    Isotyp
    IgG
    Top Product
    Discover our top product DGCR8 Primärantikörper
  • Applikationshinweise
    WB 1:1000-3000, IF/ICC, ELISA(peptide) 1:20000-1:40000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20 °C. Stable for 12 months from date of receipt.
    Haltbarkeit
    12 months
  • Target
    DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
    Andere Bezeichnung
    DGCR8 (DGCR8 Produkte)
    Hintergrund

    Description: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).

    Gene: DGCR8

    Molekulargewicht
    100kDa
    Gen-ID
    54487
    UniProt
    Q8WYQ5
    Pathways
    Regulatorische RNA Pathways
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