DGCR8 Antikörper (C-Term)
-
- Target Alle DGCR8 Antikörper anzeigen
- DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
-
Bindungsspezifität
- C-Term
-
Reaktivität
- Human, Maus, Ratte
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser DGCR8 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Spezifität
- DGCR8 Antibody detects endogenous levels of total DGCR8.
- Homologie
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus
- Aufreinigung
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human DGCR8, corresponding to a region within C-terminal amino acids.
- Isotyp
- IgG
- Top Product
- Discover our top product DGCR8 Primärantikörper
-
-
- Applikationshinweise
- WB 1:1000-3000, IF/ICC, ELISA(peptide) 1:20000-1:40000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20 °C. Stable for 12 months from date of receipt.
- Haltbarkeit
- 12 months
-
- Target
- DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
- Andere Bezeichnung
- DGCR8 (DGCR8 Produkte)
- Synonyme
- MGC78846 antikoerper, DGCR8 antikoerper, gy1 antikoerper, dgcrk6 antikoerper, C22orf12 antikoerper, DGCRK6 antikoerper, Gy1 antikoerper, pasha antikoerper, D16H22S1742E antikoerper, D16H22S788E antikoerper, D16Wis2 antikoerper, N41 antikoerper, Vo59c07 antikoerper, si:ch211-106a19.4 antikoerper, wu:fc23f08 antikoerper, wu:fc38f06 antikoerper, DGCR8 microprocessor complex subunit L homeolog antikoerper, DiGeorge syndrome critical region gene 8 antikoerper, DGCR8 microprocessor complex subunit antikoerper, DGCR8, microprocessor complex subunit antikoerper, microRNA 3618 antikoerper, dgcr8.L antikoerper, DGCR8 antikoerper, dgcr8 antikoerper, Dgcr8 antikoerper, MIR3618 antikoerper
- Hintergrund
-
Description: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).
Gene: DGCR8
- Molekulargewicht
- 100kDa
- Gen-ID
- 54487
- UniProt
- Q8WYQ5
- Pathways
- Regulatorische RNA Pathways
-