DNA2 Antikörper (Internal Region)
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- Target Alle DNA2 Antikörper anzeigen
- DNA2 (DNA Replication Helicase 2 Homolog (DNA2))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser DNA2 Antikörper ist unkonjugiert
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Applikation
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Spezifität
- DNA2L Antibody detects endogenous levels of total DNA2L.
- Homologie
- Pig,Bovine,Horse,Sheep,Rabbit,Dog
- Aufreinigung
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human DNA2L, corresponding to a region within the internal amino acids.
- Isotyp
- IgG
- Top Product
- Discover our top product DNA2 Primärantikörper
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- Applikationshinweise
- WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20 °C. Stable for 12 months from date of receipt.
- Haltbarkeit
- 12 months
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- Target
- DNA2 (DNA Replication Helicase 2 Homolog (DNA2))
- Andere Bezeichnung
- DNA2 (DNA2 Produkte)
- Synonyme
- DNA2L antikoerper, Dna2l antikoerper, E130315B21Rik antikoerper, PEOA6 antikoerper, hDNA2 antikoerper, XDna2 antikoerper, dna2-A antikoerper, DNA replication helicase/nuclease 2 antikoerper, DNA replication helicase/nuclease 2 S homeolog antikoerper, DNA2 antikoerper, Dna2 antikoerper, dna2.S antikoerper
- Hintergrund
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Description: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
Gene: DNA2
- Molekulargewicht
- 120 kDa
- Gen-ID
- 1763
- UniProt
- P51530
- Pathways
- Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA
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