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ELF1 Antikörper (C-Term)

ELF1 Reaktivität: Human WB, ELISA, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6261511
  • Target Alle ELF1 Antikörper anzeigen
    ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
    Bindungsspezifität
    • 15
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 44
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 40
    • 3
    • 1
    Kaninchen
    Klonalität
    • 43
    • 1
    Polyklonal
    Konjugat
    • 24
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ELF1 Antikörper ist unkonjugiert
    Applikation
    • 35
    • 13
    • 13
    • 11
    • 10
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Spezifität
    ELF1 Antibody detects endogenous levels of total ELF1.
    Homologie
    Horse
    Aufreinigung
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human ELF1, corresponding to a region within C-terminal amino acids.
    Isotyp
    IgG
    Top Product
    Discover our top product ELF1 Primärantikörper
  • Applikationshinweise
    WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #104221 (Western Blotting)
    'Independent Validation' Siegel
    by
    Dittmann Lab, Microbiology Department, NYU Langone Health, NYU School of Medicine
    No.
    #104221
    Datum
    28.10.2019
    Antigen
    ELF1
    Chargennummer
    4318850
    Validierte Anwendung
    Western Blotting
    Positivkontrolle

    A549 (endogenous ELF1), ELF1 recombinant protein

    Negativkontrolle
    Bewertung

    Passed. ABIN6261511 recognizes recombinant and endogenous human ELF1. The antibody does also reveal an additional larger MW protein band in A549 cell lysates.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN6261511
    Sekundärantikörper
    goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234)
    Full Protocol
    • Grow A549 cells (ATCC, CCL-185) in DMEM medium (Corning, 10-013-CV) supplemented with 10% FBS (Atlanta biologicals, S11150) and Penicillin-Streptomycin (Corning, 30-002-CI), at 37°C and 5% CO2 to 3x104 cells/cm2 in 2ml on a 6 well plate (Costar, 3516).
    • Wash cells once with 1x PBS treat with trypsin (Corning, 25-053-CI).
    • Take cells up in growth medium.
    • Wash cells once with 1x PBS.
    • Resuspend approximately 2.05x105cells/well in 50-100µl 1x LDS sample buffer (Life Technologies, B0007). As positive control, take 20µg recombinant ELF1 (Abnova, H00001997-P01) in 1x LDS sample buffer.
    • Denature samples for 3min at 95°C and subsequently keep them on ice.
    • Separate samples on a Bolt 4-12% Bis-Tris Plus Gel (Invitrogen, NW04122) in an electrophoresis chamber (Mini Gel Tank, Invitrogen, A25977) for 30min at 80V and then for 60min at 120V.
    • Transfer proteins onto nitrocellulose membrane (Invitrogen, IB23002) using an iBlot 2 Gel Transfer Device (Invitrogen).
    • Block the membrane with TBS containing 0.05% Tween (TBST) containing 5% skim milk for 1h at RT.
    • Incubation with primary
      • rabbit anti-ELF1 antibody (antibodies-online, ABIN6261511, 4318850) diluted 1:1000 in TBST containing 0.5% skim milk ON at 4°C.
      • loading control mouse anti-beta actin antibody (Invitrogen, MA5-15739) diluted 1:5000 in TBST containing 0.5% skim milk ON at 4°C.
    • Wash membrane 3x for 10min with TBST.
    • Incubation with secondary
      • goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
      • goat anti-mouse HRP-conjugated antibody (Invitrogen, G21040) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
    • Wash membrane 3x for 10min with TBST.
    • Reveal protein bands using SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific, 34075) and a ChemiDoc MP Imaging System (Bio-Rad).
    Anmerkungen
    • ABIN6261511 reveals a protein with an apparent molecular weight of approximately 62kDa. The expected molecular weight for endogenous human ELF1 is approximately 67kDa. The antibody also reveals a recombinant GST-tagged protein at the expected molecular weight (theoretical MW 93.9kDa).

    • Other ELF1-antibody dilutions were tested, but a dilution of 1:2500 was found optimal in terms of minimal background and strength of signal.

  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20 °C. Stable for 12 months from date of receipt.
    Haltbarkeit
    12 months
  • Target
    ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
    Andere Bezeichnung
    ELF1 (ELF1 Produkte)
    Synonyme
    zef1 antikoerper, fb05d07 antikoerper, wu:fb05d07 antikoerper, Elf-1 antikoerper, Sts1 antikoerper, mElf-1 antikoerper, p70 antikoerper, xelf1 antikoerper, elf-1 antikoerper, E74 like ETS transcription factor 1 antikoerper, E74-like ETS transcription factor 1 antikoerper, E74-like factor 1 antikoerper, E74 like ETS transcription factor 1 L homeolog antikoerper, E74 like ETS transcription factor 1 S homeolog antikoerper, ELF1 antikoerper, elf1 antikoerper, Elf1 antikoerper, elf1.L antikoerper, elf1.S antikoerper
    Hintergrund

    Description: Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.

    Gene: ELF1

    Molekulargewicht
    67kDa
    Gen-ID
    1997
    UniProt
    P32519
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