GJB2 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN626152

Produktdetails anti-GJB2 Antikörper

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GJB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: IHC-plus™

Spezifität: Reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5kD. Although the polyclonal anti-Cx26 antibody (feline. No. 71-0500) raised against the same sequence as this monoclonal anti-Cx26 antibody cross-reacts with Cx30, this monoclonal anti-Cx26 appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions. Rat, mouse and human. Reactivity of this antibody on Western blots has been confirmed by using extracts from mouse liver, mouse brain and rat brain.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26. (4,5).

Type of Immunogen: Synthetic peptide

Klon: CX-12H10

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-Fr, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Immunohistochemistry (Frozen): 1-2 μg/mL. Western Blot: 1 μg/mL. Reactivity confirmed using extracts from mouse liver, mouse brain and rat brain.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Details zu GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Andere Bezeichnung: GJB2 / CX26 / Connexin 26 (GJB2 Produkte)

Hintergrund: Name/Gene ID: GJB2
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJB2, Connexin 26, Connexin-26, DFNB1A, DFNA3, DFNB1, Gap junction protein beta 2, KID, HID, PPK, NSRD1, CX26, DFNA3A

Gen-ID: 2706

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization