PARP1 Antikörper (Internal Region)

Produktnummer ABIN6264040

Produktdetails anti-PARP1 Antikörper

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PARP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: PARP1 Antibody detects endogenous levels of total PARP1.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PARP1, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Referenzen für anti-PARP1 Antikörper (ABIN6264040)

Liu, Geng, Zhang, Wang, Zhang, Duan, Zhang: "Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway." in: Marine drugs, Vol. 16, Issue 3, (2018) (PubMed).

Details zu PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: PARP1 (PARP1 Produkte)

Synonyme: ADPRT antikoerper, ADPRT 1 antikoerper, ADPRT1 antikoerper, ARTD1 antikoerper, PARP antikoerper, PARP-1 antikoerper, PPOL antikoerper, pADPRT-1 antikoerper, Adprt antikoerper, Parp-1 antikoerper, 5830444G22Rik antikoerper, AI893648 antikoerper, Adprp antikoerper, Adprt1 antikoerper, C80510 antikoerper, parp-1 antikoerper, sPARP-1 antikoerper, BEST:LD21673 antikoerper, CG17685 antikoerper, CG17696 antikoerper, CG17718 antikoerper, CG40411 antikoerper, D.PARP antikoerper, Dm.pARTa antikoerper, Dmel\\CG40411 antikoerper, LD21673.3prime antikoerper, PARP1 antikoerper, dPARP antikoerper, parp antikoerper, adprt1 antikoerper, padprt-1 antikoerper, ppol antikoerper, si:dkey-206f10.3 antikoerper, wu:fc60f12 antikoerper, zgc:110092 antikoerper, poly(ADP-ribose) polymerase 1 antikoerper, poly (ADP-ribose) polymerase 1 antikoerper, poly (ADP-ribose) polymerase family, member 1 antikoerper, Poly-(ADP-ribose) polymerase antikoerper, poly(ADP-ribose) polymerase 1 L homeolog antikoerper, PARP1 antikoerper, Parp1 antikoerper, Parp antikoerper, parp1.L antikoerper, parp1 antikoerper

Hintergrund:

Description: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1, HPF1 conferring serine specificity (PubMed:28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).

Gene: PARP1

Molekulargewicht: 113kDa

Gen-ID: 142

UniProt: P09874

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location