POLH Antikörper (Internal Region)
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- Target Alle POLH Antikörper anzeigen
- POLH (Polymerase (DNA Directed), eta (POLH))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser POLH Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Spezifität
- POLH Antibody detects endogenous levels of total POLH.
- Homologie
- Sheep
- Aufreinigung
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human POLH, corresponding to a region within the internal amino acids.
- Isotyp
- IgG
- Top Product
- Discover our top product POLH Primärantikörper
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- Applikationshinweise
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20 °C. Stable for 12 months from date of receipt.
- Haltbarkeit
- 12 months
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- Target
- POLH (Polymerase (DNA Directed), eta (POLH))
- Andere Bezeichnung
- POLH (POLH Produkte)
- Synonyme
- POLH antikoerper, fc50a12 antikoerper, wu:fc50a12 antikoerper, zgc:136881 antikoerper, zgc:152642 antikoerper, RAD30 antikoerper, RAD30A antikoerper, XP-V antikoerper, XPV antikoerper, polymerase (DNA directed), eta S homeolog antikoerper, DNA polymerase eta antikoerper, polymerase (DNA directed), eta antikoerper, putative DNA polymerase eta antikoerper, polymerase (DNA directed), eta (RAD 30 related) antikoerper, polh.S antikoerper, POLH antikoerper, polh antikoerper, Tb10.70.6190 antikoerper, LINJ_21_0690 antikoerper, Polh antikoerper, LOC100443019 antikoerper
- Hintergrund
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Description: DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940). Targets POLI to replication foci (PubMed:12606586).
Gene: POLH
- Molekulargewicht
- 78kDa
- Gen-ID
- 5429
- UniProt
- Q9Y253
- Pathways
- DNA Reparatur
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