SKP1 Antikörper (Internal Region)

Produktnummer ABIN6265102

Produktdetails anti-SKP1 Antikörper

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SKP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Skp1 Antibody detects endogenous levels of total Skp1.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Skp1, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Andere Bezeichnung: SKP1 (SKP1 Produkte)

Synonyme: EMC19 antikoerper, OCP-II antikoerper, OCP2 antikoerper, SKP1A antikoerper, TCEB1L antikoerper, p19A antikoerper, 15kDa antikoerper, 2610043E24Rik antikoerper, 2610206H23Rik antikoerper, SKP1 antikoerper, Tceb1l antikoerper, p19Skp1 antikoerper, Skp1a antikoerper, skp1a antikoerper, zgc:73186 antikoerper, ocp2 antikoerper, emc19 antikoerper, ocp-ii antikoerper, tceb1l antikoerper, ARABIDOPSIS SKP1 HOMOLOGUE 1 antikoerper, ASK1 antikoerper, ATSKP1 antikoerper, S phase kinase-associated protein 1 antikoerper, T4O12.17 antikoerper, T4O12_17 antikoerper, UFO INTERACTING PROTEIN 1 antikoerper, UIP1 antikoerper, ocp-2 antikoerper, S-phase kinase associated protein 1 antikoerper, S-phase kinase-associated protein 1 antikoerper, S-phase kinase-associated protein 1 pseudogene antikoerper, S-phase kinase-associated protein 1A antikoerper, S-phase kinase-associated protein 1 S homeolog antikoerper, S phase kinase-associated protein 1 antikoerper, SKP1 antikoerper, skp1 antikoerper, LOC100340043 antikoerper, Skp1a antikoerper, Skp1 antikoerper, skp1.S antikoerper

Hintergrund:

Description: Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2 (PubMed:25704143). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22', the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.

Gene: SKP1

Molekulargewicht: 18kDa

Gen-ID: 6500

UniProt: P63208

Pathways: Zellzyklus, Hedgehog Signalweg, Mitotic G1-G1/S Phases