RENT1/UPF1 Antikörper

Produktnummer ABIN6266288

Produktdetails anti-RENT1/UPF1 Antikörper

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RENT1/UPF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: UPF1 Antibody detects endogenous levels of total UPF1

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthetic peptide of human UPF1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C.Stable for 12 months from date of receipt

Haltbarkeit: 12 months

Details zu RENT1/UPF1

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Andere Bezeichnung: UPF1 (UPF1 Produkte)

Synonyme: HUPF1 antikoerper, NORF1 antikoerper, RENT1 antikoerper, pNORF1 antikoerper, smg-2 antikoerper, B430202H16Rik antikoerper, PNORF-1 antikoerper, Rent1 antikoerper, Upflp antikoerper, rent1 antikoerper, wu:fi40f07 antikoerper, wu:fj48a01 antikoerper, zgc:55472 antikoerper, Tb05.3C6.50 antikoerper, AO090012000584 antikoerper, hupf1 antikoerper, norf1 antikoerper, pnorf1 antikoerper, upf1 antikoerper, UPF1, RNA helicase and ATPase antikoerper, UPF1 regulator of nonsense transcripts homolog (yeast) antikoerper, upf1 regulator of nonsense transcripts homolog (yeast) antikoerper, regulator of nonsense transcripts 1 antikoerper, Regulator of nonsense transcripts 1 antikoerper, UPF1 regulator of nonsense transcripts homolog S homeolog antikoerper, UPF1 antikoerper, Upf1 antikoerper, upf1 antikoerper, Tc00.1047053511317.30 antikoerper, Tb927.5.2140 antikoerper, TVAG_453890 antikoerper, TVAG_237840 antikoerper, AOR_1_1018194 antikoerper, HPB8_739 antikoerper, smg-2 antikoerper, upf1.S antikoerper

Hintergrund:

Description: RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation, its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S, the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.

Gene: UPF1

Molekulargewicht: 123kDa

Gen-ID: 5976

UniProt: Q92900

Pathways: SARS-CoV-2 Protein Interaktom