MEK1/2 Antikörper (pSer218, pSer222)
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- Target Alle MEK1/2 (MAP2K1/2) Produkte
- MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))
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Bindungsspezifität
- AA 160-240, pSer218, pSer222
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser MEK1/2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Verwendungszweck
- MEK-1/2 is a protein encoded by the MAP2K1 gene which is approximately 43,4 kDa. MEK-1/2 is localised to the cytoplasm and nucleus. It is involved in RET signalling, activated TLR4 signalling, the IL-2 pathway, regulation of lipid metabolism and insulin signalling-generic cascades. It acts as an integration point for multiple biochemical signals such as proliferation, differentiation, transcription regulation and development. It is also a dual-specificity kinase known for its involvement in the ERK pathway by activation of ERK1 and ERK2. MEK-1/2 is widely expressed with low levels in the brain. Mutations in the MAP2K1 gene may result in Cardiofaciocutaneous syndrome. STJ90327 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous MEK-1/2 protein which only binds about S218/222 when S218/222 is phosphorylated.
- Spezifität
- Phospho-MEK-1/2 (S218/222) Polyclonal Antibody detects endogenous levels of MEK-1/2 protein only when phosphorylated at S218/222.
- Produktmerkmale
- Rabbit polyclonal to Phospho-MEK-1/2 (S218/222).
- Aufreinigung
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Synthesized peptide derived from human MEK-1/2 around the phosphorylation site of S218/222.
- Isotyp
- IgG
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- Applikationshinweise
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WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000 - Kommentare
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Widely expressed, with extremely low levels in brain.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- Target
- MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))
- Andere Bezeichnung
- MEK-1/2 (MAP2K1/2 Produkte)
- Molekulargewicht
- 48 kDa
- Gen-ID
- 5604
- UniProt
- Q02750
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