IGLV1-51 Antikörper

Produktnummer ABIN6296641

Produktdetails anti-IGLV1-51 Antikörper

Target: IGLV1-51 (Immunoglobulin lambda Variable 1-51 (IGLV1-51))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IGLV1-51 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Verwendungszweck: Mouse anti-Human Lambda Light Chain Antibody [Sodium Azide Free]

Spezifität: Cell Surface, Cytoplasmic and Secreted

Aufreinigung: IgG purified from human serum was used as the immunogen for this Lambda Light Chain antibody.

Immunogen: IgG purified from human serum was used as the immunogen for this Lambda Light Chain antibody.

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu IGLV1-51

Target: IGLV1-51 (Immunoglobulin lambda Variable 1-51 (IGLV1-51))

Andere Bezeichnung: Immunoglobulin lambda variable 1-51 (IGLV1-51 Produkte)

Synonyme: IGLV151 antikoerper, V1-19 antikoerper, immunoglobulin lambda variable 1-51 antikoerper, IGLV1-51 antikoerper

Hintergrund:

Target Description: Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte. In a healthy individual, the total kappa to lambda ratio is roughly 3:1 in serum (measuring intact whole antibodies) or 1:1.5 if measuring free light chains, with a highly divergent ratio indicative of neoplasm.Individual B-cells in lymphoid tissue possess either kappa or lambda light chains, but never both together. Specific rearrangement of lambda light chain of immunoglobulins can lead to loss of some protein coding genes, which does not seem to be functionally relevant (while functionally relevant miR-650 can be overexpressed). Using immunohistochemistry, it is possible to determine the relative abundance of B-cells expressing kappa and lambda light chains. If the lymph node or similar tissue is reactive, or otherwise benign, it should possess a mixture of kappa positive and lambda positive cells. If, however, one type of light chain is significantly more common than the other, the cells are likely all derived from a small clonal population, which may indicate a malignant condition, such as B-cell lymphoma. [Wiki]

Gene Symbol: IGLV1-51///IGLC1

Gen-ID: 3535

UniProt: P01701, P0CG04