CD3 Antikörper

Produktnummer ABIN638428

Produktdetails anti-CD3 Antikörper

Target: CD3

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Cytometry by Time of Flight (CyTOF)

Spezifität: The antibody MEM-57 reacts with an extracellular epitope on gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Human thymocytes and T lymphocytes.

Klon: MEM-57

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation: The antibody MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28- kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7).
Functional application: The antibody MEM-57 has strong mitogenic effect on peripheral T lymphocytes, it reacts strongly with gamma/delta T lymphocytes.
Flow cytometry: Recommended dilution: 1-4 μg/mL, positive control: peripheral blood lymphocytes, JURKAT human leukemia T cell line.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel: Azide free

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD3 Antikörper (ABIN638428)

Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, (2012) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

Panyi, Bagdány, Bodnár, Vámosi, Szentesi, Jenei, Mátyus, Varga, Waldmann, Gáspar, Damjanovich: "Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2592-7, (2003) (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).

Dave, Cao, Browne, Alarcon, Fernandez-Miguel, Lafaille, de la Hera, Tonegawa, Kappes: "CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage." in: The EMBO journal, Vol. 16, Issue 6, pp. 1360-70, (1997) (PubMed).

Soucek, Hilgert, Budová, Lindnerová: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." in: Neoplasma, Vol. 41, Issue 2, pp. 75-81, (1994) (PubMed).

Hilgert, Fran?k, Stefanová, Kaslík, Jirka, Kristofová, Rossmann, Soucek, Horejsi: "Therapeutic in vivo use of the A1-CD3 monoclonal antibody." in: Transplantation, Vol. 55, Issue 2, pp. 435-8, (1993) (PubMed).

Soucek, Chudomel, Hrubá, Lindnerová: "Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3." in: Neoplasma, Vol. 38, Issue 1, pp. 33-41, (1991) (PubMed).

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3 (CD3 Produkte)

Synonyme: CD3 antikoerper, AI504783 antikoerper, CD3epsilon antikoerper, T3e antikoerper, cd3-gamma antikoerper, cd3g antikoerper, cd3g-a antikoerper, cd3g/d antikoerper, cd3gd antikoerper, t3g antikoerper, CD3e molecule antikoerper, CD3d molecule antikoerper, CD3 antigen, epsilon polypeptide antikoerper, CD3g molecule S homeolog antikoerper, CD3E antikoerper, CD3D antikoerper, Cd3e antikoerper, cd3g.S antikoerper

Hintergrund: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

Gen-ID: 916

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway