Bcl-2 Antikörper (AA 41-54) (PE)

Produktnummer ABIN638444

Produktdetails anti-Bcl-2 Antikörper (PE)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Bindungsspezifität: AA 41-54

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Bcl-2 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa intracellular protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.

Keine Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2

Klon: Bcl-2-100

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Intracellular staining.

Kommentare:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-Bcl-2 Antikörper (PE) (ABIN638444)

Joubert, Marais, Maritz: "Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopi?a Electronica ... et. al, Vol. 33, Issue 1, pp. 67-70, (2009) (PubMed).

Laforge, Petit, Estaquier, Senik: "Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef." in: Journal of virology, Vol. 81, Issue 20, pp. 11426-40, (2007) (PubMed).

Gugasyan, Christou, OReilly, Strasser, Gerondakis: "Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA." in: Cell death and differentiation, Vol. 13, Issue 7, pp. 1235-7, (2006) (PubMed).

Laflamme, Israël-Assayag, Cormier: "Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis." in: The European respiratory journal, Vol. 21, Issue 2, pp. 225-31, (2003) (PubMed).

Soilu-Hänninen, Ekert, Bucci, Syroid, Bartlett, Kilpatrick: "Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 12, pp. 4828-38, (1999) (PubMed).

Details zu Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Andere Bezeichnung: Bcl2 (BCL2 Produkte)

Synonyme: Bcl-2 antikoerper, PPP1R50 antikoerper, bcl-2 antikoerper, AW986256 antikoerper, C430015F12Rik antikoerper, D630044D05Rik antikoerper, D830018M01Rik antikoerper, BCL-2 antikoerper, Bcl2 antikoerper, BCL2, apoptosis regulator antikoerper, B cell leukemia/lymphoma 2 antikoerper, B-cell CLL/lymphoma 2a antikoerper, B-cell CLL/lymphoma 2 protein antikoerper, BCL2 antikoerper, Bcl2 antikoerper, bcl2a antikoerper, BCL-2 antikoerper

Hintergrund: BCL2 apoptosis regulator,Bcl2 (B cell chronic lymphatic leukemia protein 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.,Bcl-2, PPP1R50

Gen-ID: 596

UniProt: P10415

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagie, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling