LAMP1 Antikörper (PE)

Produktnummer ABIN638446

Produktdetails anti-LAMP1 Antikörper (PE)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reaktivität: Human, Maus, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LAMP1 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody H4A3 recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.

Kreuzreaktivität (Details): Human, Non-Human Primates, Mouse

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human PBMC

Klon: H4A3

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular and extracellular staining.

Kommentare:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-LAMP1 Antikörper (PE) (ABIN638446)

Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, (2012) (PubMed).

Mao, Tu, Liu, Qin, Zheng, Chan, Lam, Peiris, Lau: "Inhibition of human natural killer cell activity by influenza virions and hemagglutinin." in: Journal of virology, Vol. 84, Issue 9, pp. 4148-57, (2010) (PubMed).

Yu, Gallegos, Marches, Zurawski, Ramilo, García-Sastre, Banchereau, Palucka: "Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines." in: Blood, Vol. 112, Issue 9, pp. 3671-8, (2008) (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, (2007) (PubMed).

Tomescu, Chehimi, Maino, Montaner: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, (2007) (PubMed).

Marcenaro, Gallo, Martini, Santoro, Griffiths, Aricó, Moretta, Pende: "Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease." in: Blood, Vol. 108, Issue 7, pp. 2316-23, (2006) (PubMed).

Deetz, Hebbeler, Propp, Cairo, Tikhonov, Pauza: "Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys." in: Infection and immunity, Vol. 74, Issue 8, pp. 4505-11, (2006) (PubMed).

Furuta, Ikeda, Nakayama, Nakamura, Tanaka, Hamasaki, Himeno, Hamilton, August: "Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases." in: The American journal of pathology, Vol. 159, Issue 2, pp. 449-55, (2001) (PubMed).

Mane, Marzella, Bainton, Holt, Cha, Hildreth, August: "Purification and characterization of human lysosomal membrane glycoproteins." in: Archives of biochemistry and biophysics, Vol. 268, Issue 1, pp. 360-78, (1989) (PubMed).

Details zu LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Andere Bezeichnung: CD107a (LAMP1 Produkte)

Synonyme: CD107a antikoerper, LAMPA antikoerper, LGP120 antikoerper, AI196048 antikoerper, Lamp-1 antikoerper, LEP100 antikoerper, LAMP-1 antikoerper, LGP-A antikoerper, Lgpa antikoerper, Lysosome-associated membrane protein 1 antikoerper, cd107a antikoerper, lamp-1 antikoerper, lamp1 antikoerper, lampa antikoerper, lgp120 antikoerper, rexp76 antikoerper, lysosomal associated membrane protein 1 antikoerper, lysosomal-associated membrane protein 1 antikoerper, lysosomal-associated membrane protein 1 L homeolog antikoerper, LAMP1 antikoerper, Lamp1 antikoerper, lamp1.L antikoerper

Hintergrund: Lysosomal associated membrane protein 1,CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2 % of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.,LAMP-1, LAMPA

Gen-ID: 3916

UniProt: P11279

Pathways: Autophagie