HDAC2 Antikörper (Internal Region)

Produktnummer ABIN6391348

Produktdetails anti-HDAC2 Antikörper

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HDAC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: Hdac2 (mouse)

Sequenz: PEDAVHEDSG DE

Spezifität: This antibody imay cross-react with HDAC1

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 55-60 kDa band observed in nuclear lysates of cell line HEK293 (calculated MW of 55.3 kDa according to NP_032255.2). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:2000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U251 cells and additionally in the membrane and cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu HDAC2

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Andere Bezeichnung: Hdac2 (HDAC2 Produkte)

Synonyme: HD2 antikoerper, RPD3 antikoerper, YAF1 antikoerper, D10Wsu179e antikoerper, Yy1bp antikoerper, mRPD3 antikoerper, HISTONE DEACETYLASE antikoerper, T1N24.9 antikoerper, T1N24_9 antikoerper, histone deacetylase 2 antikoerper, histone deacetylase 2 antikoerper, histone deacetylase 2 S homeolog antikoerper, HDAC2 antikoerper, Hdac2 antikoerper, hdac2.S antikoerper, HDA2 antikoerper

Hintergrund: Hdac2, histone deacetylase 2, D10Wsu179e, YAF1, Yy1bp, mRPD3, HD2, OTTMUSP00000022803, YY1 transcription factor-binding protein

Gen-ID: 3066, 15182, 84577

NCBI Accession: NP_032255

Pathways: Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion