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Hsc70 Antikörper (N-Term)

Name: Hsc70 Antikörper (N-Term)
SKU: ABIN650754
Price: 467.5 EUR
Availability: InStock

HSPA8 Reaktivität: Human WB, IF, IHC (p), FACS Wirt: Kaninchen Polyclonal RB17655 unconjugated

Western blot analysis of lysates from A431,Hela,mouse NIH/3T3, rat H-4-II-E cell line (from left to right),using HS Antibody (N-term) (ABIN650754 and ABIN2839488). (Hsc70 Antikörper (N-Term))

Formalin-fixed and paraffin-embedded human brain tissue reacted with HS Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (Hsc70 Antikörper (N-Term))

IHC (p)

Confocal immunofluorescent analysis of HS Antibody (N-term) (ABIN650754 and ABIN2839488) with cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (Hsc70 Antikörper (N-Term))

HS Antibody (N-term) (ABIN650754 and ABIN2839488) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). (Hsc70 Antikörper (N-Term))

FACS

4 Abbildungen

(2 Referenzen)

Produktnummer ABIN650754

Datenblatt (PDF)

Target Alle Hsc70 (HSPA8) Antikörper anzeigen

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Bindungsspezifität
14

6

6

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 82-110, N-Term
Reaktivität
83

48

44

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
Human
Wirt
71

28

7

2
Kaninchen
Passende Sekundärantikörper
Klonalität
68

39
Polyklonal
Konjugat
60

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Hsc70 Antikörper ist unkonjugiert
Applikation
94

51

41

30

29

28

15

13

11

11

11

5

4

2

2

2

2
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Homologie

B, C, Ha, Hs, M, Rat

Aufreinigung

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human HSPA8.

Klon

RB17655

Isotyp

Ig Fraction

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Applikationshinweise

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Fitter, Gronthos, Ooi, Zannettino: "The Mesenchymal Precursor Cell Marker Antibody STRO-1 Binds to Cell Surface Heat Shock Cognate 70." in: Stem cells (Dayton, Ohio), Vol. 35, Issue 4, pp. 940-951, (2017) (PubMed).

Li, Li, Wang, Cui, Gong, Qu, Zhong, Zhou, Zhou, Gao, Li: "Quantitative proteomic study of human prostate cancer cells with different metastatic potentials." in: International journal of oncology, Vol. 48, Issue 4, pp. 1437-46, (2016) (PubMed).
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Andere Bezeichnung

HSPA8 (HSPA8 Produkte)

Hintergrund

HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.

Molekulargewicht

70898

Gen-ID

3312

NCBI Accession

NP_006588, NP_694881

UniProt

P11142