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His Tag Antikörper

Name: His Tag Antikörper
SKU: ABIN652278
Price: 445.5 EUR
Availability: InStock

Reaktivität: Human WB Wirt: Kaninchen Polyclonal RB19960 unconjugated

All lanes : Anti-His Tag Antibody at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: 293T/17 transfected with 12tag whole cell lysate Lane 3: 12tag recombinant protein Lysates/proteins at 1 μg per lane. (His Tag Antikörper)

Anti-His Tag at 1:8000 dilution + 12tag recombination protein Lysates/proteins at 20 ng per lane. (His Tag Antikörper)

2 Abbildungen

(1 reference)

Produktnummer ABIN652278

Datenblatt (PDF)

Target Alle His Tag Antikörper anzeigen

His Tag
Reaktivität
20

5

4

1

1

1
Human
Wirt
92

59

11

9

5

4

1

1
Kaninchen
Passende Sekundärantikörper
Klonalität
94

75

10
Polyklonal
Konjugat
78

22

13

13

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser His Tag Antikörper ist unkonjugiert
Applikation
140

91

39

27

25

18

15

14

14

13

13

7

7

5

5

4

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB)

Aufreinigung

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

Poly-HIS peptide were used to produced this antibody.

Klon

RB19960

Isotyp

Ig Fraction

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anti-His Tag antibody
WB, ELISA, IP, IHC, FACS Wirt: Maus Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS Wirt: Maus Monoclonal 6G2A9 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP Wirt: Maus Monoclonal 33D10-D2 HRP

anti-His Tag (C-Term) antibody
WB, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP Wirt: Maus Monoclonal 33D10-D2 Biotin

anti-His Tag antibody
WB, ELISA, IP, DB, ISt Wirt: Maus Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, FM, FLISA Wirt: Maus Monoclonal 33D10-D2 DyLight 649

Applikationshinweise

WB: 1:8000. WB: 1:1000. WB: 1:1000

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Jiang, Zhao, Xiong, Wang, He, Wang, Chen, Geng, Huang, Ouyang, Lai: "Immune responses of channel catfish following the stimulation of three recombinant flagellins of Yersinia ruckeri in vitro and in vivo." in: Developmental and comparative immunology, Vol. 73, pp. 61-71, (2018) (PubMed).
Target

His Tag

Andere Bezeichnung

6 X His Tag (His Tag Produkte)

Substanzklasse

Tag

Hintergrund

Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.