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SP1 Antikörper (C-Term)

Name: SP1 Antikörper (C-Term)
SKU: ABIN655398
Price: 467.5 EUR
Availability: InStock

SP1 Reaktivität: Human WB, IF, IHC (p), FACS Wirt: Kaninchen Polyclonal RB17350 unconjugated

Anti-SP1 Antibody (C-term ) at 1:500 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. (SP1 Antikörper (C-Term))

SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). (SP1 Antikörper (C-Term))

FACS

Confocal immunofluorescent analysis of SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (SP1 Antikörper (C-Term))

SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) western blot analysis in K562 cell line lysates (35 μg/lane). (SP1 Antikörper (C-Term))

SP1 Antibody (C-term ) (ABIN655398 and ABIN2844945) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. (SP1 Antikörper (C-Term))

IHC (p)

5 Abbildungen

(1 reference)

Produktnummer ABIN655398

Datenblatt (PDF)

Target Alle SP1 Antikörper anzeigen

SP1 (Sp1 Transcription Factor (SP1))
Bindungsspezifität
21

12

10

6

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 677-707, C-Term
Reaktivität
88

60

39

11

8

7

6

5

5

5

4

2

2

2

1
Human
Wirt
90

13

3
Kaninchen
Passende Sekundärantikörper
Klonalität
83

23
Polyklonal
Konjugat
72

4

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Dieser SP1 Antikörper ist unkonjugiert
Applikation
91

46

45

37

21

17

14

13

11

8

4

3

3

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Homologie

M, Rat

Aufreinigung

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 677-707 amino acids from the C-terminal region of human SP1.

Klon

RB17350

Isotyp

Ig Fraction
anti-Sp1 Transcription Factor (SP1) (AA 89-198) antibody
SP1 Reaktivität: Human WB, ELISA, IF, IHC (p) Wirt: Maus Monoclonal 1A5 unconjugated

anti-Sp1 Transcription Factor (SP1) (AA 522-618) antibody
SP1 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 4B11 unconjugated

anti-Sp1 Transcription Factor (SP1) (AA 89-198) antibody
SP1 Reaktivität: Human WB, ELISA, IF, IHC (p) Wirt: Maus Monoclonal 4H6 unconjugated

anti-Sp1 Transcription Factor (SP1) (C-Term) antibody
SP1 Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Sp1 Transcription Factor (SP1) (pThr453) antibody
SP1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Sp1 Transcription Factor (SP1) antibody
SP1 Reaktivität: Human WB, IHC, IF, ChIP Wirt: Kaninchen Monoclonal unconjugated

anti-Sp1 Transcription Factor (SP1) (pThr739) antibody
SP1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Sp1 Transcription Factor (SP1) (AA 420-620) antibody
SP1 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Sp1 Transcription Factor (SP1) (AA 89-199) antibody
SP1 Reaktivität: Human, Maus WB, ELISA, IF Wirt: Maus Monoclonal 4H6 unconjugated

anti-Sp1 Transcription Factor (SP1) (AA 677-707) antibody
SP1 Reaktivität: Human WB, ELISA, IHC, IF, FACS Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

IF: 1:10~50. WB: 1:500. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Zhang, Wang, Qin, Liu, Sun, Liu, Kuang, Jiang, Zhang: "cis-Acting elements and trans-acting factors in the transcriptional regulation of raf kinase inhibitory protein expression." in: PLoS ONE, Vol. 8, Issue 12, pp. e83097, (2014) (PubMed).
Target

SP1 (Sp1 Transcription Factor (SP1))

Andere Bezeichnung

SP1 (SP1 Produkte)

Hintergrund

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Molekulargewicht

80693

Gen-ID

6667

NCBI Accession

NP_001238754, NP_003100, NP_612482

UniProt

P08047

Pathways

Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction